p16、RAR-β和APC基因甲基化联合检测对恶性浆膜腔积液的诊断价值

Diagnostic value of combined detection of p16,RAR-beta and APC genes methylation in malignant serous effusion

目的:探讨浆膜腔积液沉渣细胞的肿瘤抑制蛋白(p16)、视黄酸受体β(RAR-β)和结肠腺瘤性息肉病(APC)基因甲基化状态检测在良、恶性浆膜腔积液中的鉴别诊断价值。方法:选取太和医院的患者浆膜腔积液患者65例,其中良性浆膜腔积液27例,恶性浆膜腔积液38例。运用甲基化特异性PCR技术对p16、RAR-β和APC基因启动子区域进行甲基化检测。结果:p16基因和RAR-β基因的甲基化频率2组比较差异无统计学意义;APC基因甲基化频率良性组为7.4%,恶性组为28.9%,2组比较差异有统计学意义(P<0.05);p16、RAR-β和APC基因甲基化检测的阳性率恶性组为84.2%,84.2%,76.3%,良性组为33.3%,44.4%,14.8%,2组比较差异有统计学意义(P<0.05)。结论:浆膜腔积液p16、RAR-β和APC基因甲基化检查对恶性肿瘤的早期诊断具有非常重要的诊断价值。

Objective：To investigate the differential diagnostic value of the p16,Retinoic acid receptor beta（RAR-beta）and adenomatous polyposis coli（APC）gene methylation in the serous effusion.Method：There were 27 cases of benign and 38 cases of malignant serous effusion.p16,RAR-βand APC gene methylation were Methylation-specific PCR.Result：There was not statistically different between the frequency of gene methylation p16 and RAR-β.The positive rates of APC gene methylation were 7.4% and 28.9%in the benign and malignant groups,respectively（P〈0.05）.The positive rates of p16,RAR-βand APC gene methylation were 84.2%,84.2% and76.3%in the malignant group and were 33.3%,44.4% and 14.8%in the benign group（P〈0.05）.Conclusion：P16,RAR-βand APC gene methylation examination has a very important clinical value to early diagnostic of malignant tumors.