柔肝通络汤对脑缺血大鼠外周血内皮祖细胞影响的实验研究

Effect of Rougan Tongluo decoction on peripheral endothelial progenitor cells in rats with cerebral ischemia:an experimental study

目的:观察柔肝通络汤对脑缺血大鼠内皮祖细胞(Endothelial Progenitor Cells,EPCs)的影响,探讨其改善脑缺血神经功能缺损的可能机制。方法:采用线栓法制备大脑中动脉闭塞的脑缺血大鼠模型,随机分为0.9%氯化钠注射液组、辛伐他汀组、柔肝通络汤组,每组各10只;另取10只设为假手术组。各组分别于造模成功后第1天开始灌胃给药,连续7d。采用Longa神经功能评分评价造模术后3h及给药前后各组大鼠神经功能缺损情况,于造模前及给药前后使用CD_(34)、CD_(133)双抗体标记结合流式细胞仪检测各组大鼠外周血EPCs数量。结果:各治疗组给药前Longa评分均较造模术后3h降低;给药后Longa评分柔肝通络汤组、辛伐他汀组均低于0.9%氯化钠注射液组,且柔肝通络汤组低于辛伐他汀组,差异均有统计学意义(P<0.01);急性脑缺血发生后,大鼠外周血CD_(34)+CD_(133)+EPCs数量先下降再上升,柔肝通络汤与辛伐他汀可使EPCs数量增加,且柔肝通络汤作用优于辛伐他汀。结论:柔肝通络汤可能通过增加EPCs数量及功能来促进大鼠脑缺血后神经功能的恢复。

Objective：To investigate the effect of Rougan Tongluo decoction on endothelial progenitor cells（EPCs） in rats with cerebral ischemia and its possible mechanism in improving neurological defects induced by cerebral ischemia.Methods：The suture method was used to establish a rat model of cerebral ischemia with middle cerebral artery occlusion.The rats were randomly divided into 0.9% sodium chloride injection group,simvastatin group,and Rougan Tongluo decoction group,with 10 rats in each group,and another 10 rats were selected as sham-operation group.The drugs were given by gavage from the first day after the model was established successfully for 7 consecutive days.The Longaneurological score was used to evaluate neurological defects at 3 hours after modeling and before and after administration,and CD_（34）、CD_（133） double-antibody labeling combined with flow cytometry was used to measure the number of peripheral EPCs before modeling and before and after administration.Results：All the treatment groups had a significant reduction in the Longaneurological score before administration.After administration,the Rougan Tongluo decoction group and the simvastatin group had a significantly lower score than the 0.9% sodium chloride injection group,and the Rougan Tongluo decoction group had a significantly lower score than the simvastatin group（P0.01）.After the onset of acute cerebral ischemia,the number of peripheral CD_（34）＋CD_（133）＋EPCs decreased at first and then increased;bothRougan Tongluo decoction group and simvastatin group had an increase in the number of EPCs,and the Rougan Tongluo decoction group had a significantly greater increase than thesimvastatin group.Conclusion：Rougan Tongluo decoction can promote the recovery of neurological function after cerebral ischemia in rats by increasing the number of EPCs.