赣南柑橘木虱体内黄龙病菌psy62株系原噬菌体类型多样性的研究

A study on the diversity of prophage types from Candidatus liberibacter strain psy62 in Diaphorina citri collected from Southern Jiangxi Province

为探明柑橘木虱体内黄龙病菌psy62株系中原噬菌体遗传多样性及与柑橘株系的遗传差异,本研究将利用3对引物在psy62基因组中2个噬菌体FP1和FP2的同源序列区域内对赣南地区阳性样品进行PCR扩增和测序。结果显示,赣南地区阳性样品在此基因区域内有28头柑橘木虱检测到原噬菌体,共有5种类型,分别为A,A2,B,C,C1。其中A,B的检测率相对较高;C1检测率最低,为7.1%。由此可见,在此基因位点不同地理位置的柑橘木虱原噬菌体种群有较大的遗传差异,且与美国佛罗里达的"Ca.L.asiaticus"株系,中国柑橘叶片"Ca.L.asiaticus"株系均有显著的遗传差异,这有助于进一步研究分析原噬菌体的种类差异对柑橘木虱传毒机制的影响。

In order to research on the genetic diversity of prophage region from Candidatus liberibacter strain psy62 in Diaphorina citri,and the genetic differenty between D. citri and citrus strains. For this experiment,using there PCR primers to detect positive samples of D. citri were collected from seven locality in Gannan. Initial result indicated that the percentage of type A and type B was relatively high,the percentage of type C was lowest. The study result suggested that different location of prophage in D. citri populations have larger genetic differences. And ＂Ca. L. asiaticus＂strains of Florida in the United States,Chinese ＂Ca. L. asiaticus ＂ strains of citrus are all have significant genetic differences to ＂Ca. L.asiaticus＂strains of D. citri in Gannan. It will further the research and analysis of the influences of the difference prophage species to the toxic mechanism of D. citri.