菜粉蝶osiris基因家族鉴定与发育动态

Identification and developmental pattern of osiris gene family in the cabbage butterfly Pieris rapae

osiris基因家族是昆虫特异性基因,迄今尚未在昆虫纲以外的物种中发现同源基因。本研究利用菜粉蝶转录组数据,鉴定了菜粉蝶16个osiris基因家族成员,分属11个亚家族。通过与菜粉蝶基因组比对,发现菜粉蝶osiris基因均为断裂基因,外显子数量为3-15个;通过结构域分析,发现菜粉蝶Osiris完整编码蛋白含有信号肽和一个未知功能结构域DUF1676,且多数Osiris蛋白含跨膜结构域。系统发育分析表明,osiris基因家族成员与其他昆虫种类相应成员更似直系同源,而非种内基因扩张,再次验证了osiris基因是在昆虫物种分化之前就已形成的多基因家族。发育转录组基因表达分析表明,osiris家族不同成员表达量在不同发育阶段趋势几乎完全一致,多在菜粉蝶1龄幼虫和5龄幼虫高表达,卵期、蛹期与成虫期低表达,预示着osiris基因家族不同成员转录调控机制的相似性与发育的相关性。

osiris gene family are insect-specific genes,whose homologous genes are unavailable in any other species. In current study,16 osiris genes were identified belong to 11 sub-families based on the developmental transcriptome of Pieris rapae. All these genes were found to be split genes with 3 to 15 exons by compared with the P. rapae genome. Structural domain analysis revealed that each Osiris protein contained one signal peptide and one structure domain（ DUF1676）,while most of them have a transmembrane domain. Phylogenetic analysis indicated that osiris gene had been formed before insect speciation. Analysis of different developmental stage of P. rapae transcriptomes showed that osiris gene members had similar expression pattern throughout the entire life-stage（ high expression in the first and fifth larval stages and low expression in other growth stages）. These results indicated that different members of osiris gene family may be involved in similar mechanism of transcription regulation and related with development.