全场光学相干层析三维成像技术研究

Research on full-field optical coherence tomography

首先在对全场光学相干层析(FFOCT)系统的数学建模和理论分析基础上,给出FFOCT系统的轴向和平面分辨率;然后采用全场光学相干层析三维成像技术对人体食管、子宫等器官的正常和癌变样本进行内部三维成像提取,获取了微米级高分辨率的层析图像,可以清晰显示组织的细胞间质和肌纤维结构;研究实验中把FFOCT成像的结果与传统病理切片显微图像进行了比较,FFOCT系统不仅能观察到传统病理切片可以看到的细胞块形态,而且不需要切割、染色等烦琐耗时的处理,就能观察到传统方法难以获取的深度结构。该技术可以应用于癌症的早期诊断和手术中的快速诊断。

Full-field optical coherence tomography （FFOCT） was used for imaging the interior part of an object. Firstly, the mathematical modeling and performance analysis of the FFOCT system mounted was provided. Secondly, images of several different human tissues were obtained by FFOCT system, including esophagus, uterus, etc. The images of different depths of the same tissue were shown and compared. It was the first time in China that FFOCT was used for generating depth images on human esophagus and uterus; high-resolution images had been obtained for different depths without cutting the tissue sample, in which intercellular substance and myofibril structure could be clearly identified. As the in-depth structure could be imaged without the process needed for frozen and paraffin sectioning methods, the technology could been highly valuable for early cancer diagnosis and pathological analysis in tumor research.