正交实验法优化黄柏中黄连素的酶碱法提取工艺

Optimization of Enzyme-alkali Method Extraction Process of Berberine from Cortex Phellodendri by Orthogonal Experiments

为了提高黄柏中黄连素的提取得率与效率,采用传统方法碱提法与酶解辅助提取相结合来优化黄柏中黄连素的提取工艺。以黄连素浸提率为评价指标,考察纤维素酶浓度、酶解时间、料液比及浸提时间对提取效率的影响。在单因素实验的基础上,通过正交实验分析各因素的作用大小以及它们之间的交互作用。优选出黄柏中黄连素的酶碱法提取最佳工艺条件为:纤维素酶浓度15‰、酶解时间1 h、料液比1∶7、浸提时间7 h,在该条件下黄连素的浸提率可达8.99%。该提取工艺浸提率高,成本低,稳定性好,可用于生产上黄柏中黄连素的提取。

The objective of this study was to find out the optimal extraction conditions of berberine from Cortex Phello- dendri by applying the enzyme-alkali technology. Based on the single factor experiments, the orthogonal test was designed to optimize the Enzyme-alkali technology to extract berberine from cortex phellodendri with the extraction ratio as evalua- tion index. The optimal extraction conditions were as follows：the concentration of cellulase with 15‰, enzymolysis time with 1 h, substrate/liquid with 1 ： 7, and extraction time with 7 h. The process was suitble for its industrialized production with high yield,low cost and good stability.