摘要
为考察银杏叶中黄酮含量对银杏叶提取物质量、得率及黄酮提取率的影响,为银杏叶提取物的生产提供原料标准和对应的工艺参数,本文选取黄酮含量分别为1.0%、0.8%和0.6%的三批银杏叶,在保证提取物质量满足中国药典要求的前提下,探究银杏叶质量的变化对提取物大孔树脂纯化工艺条件的影响。采用统计回归方法分别建立了银杏叶提取物的黄酮含量、提取物得率及黄酮提取率与纯化乙醇浓度和体积之间的关系。研究发现银杏叶提取物中黄酮含量与乙醇浓度及洗脱液体积正相关;提取物得率与洗脱液体积负相关、与乙醇浓度的关系随银杏叶中黄酮含量而变化;低黄酮含量银杏叶的提取物质量对工艺参数最为敏感;提取物中内酯含量与银杏叶中黄酮含量呈正相关关系并与提取物中黄酮含量、洗脱液体积呈负相关关系。为保证提取物质量、工艺条件和得率的稳定,建议通过不同质量原料混合的方式使银杏叶中黄酮含量维持在1%,采用2倍量15%乙醇进行洗脱,可获得黄酮含量大于24%、内酯含量6%,得率2.6%、黄酮提取率大于66%的银杏叶提取物。
This study aims to investigate the effect of flavonoids contents in Ginkgo biloba leaves on the quality, yield and flavonoid extraction rate of extract of G. biloba leaves (EGb), and provide quality standard of raw material and cor- responding processing parameters for EGb production. G. biloba leaves with flavonoid content of 1.0%, 0.8% and 0. 6% were applied to study the effects of the quality of Ginkgo biloba leaves on the maeroporous resin purification process for ensuring the quality of EGb to meet the requirement of Chinese Pharmacopoeia. Statistic regression was used to in- vestigate the relationship between EGb properties and the concentration and volume of ethanol for purification, respec- tively. These properties include flavonoid content and yield of EGb, and extraction rate of flavonoids. The results showed that the concentration of ethanol and eluent volume was positively correlated with the content of flavonoids in EGb. The yield of EGb was negatively correlated with the eluent volume and changed with the contents of flavonoids in the leaves. The quality of EGb extracted from Ginkgo Leaves with low flavonoid concentration was most sensitive to the processing parameters. The content of laetone in EGb had a positive relation with flavonoid content in Ginkgo biloba leaves. It also had a negative correlation with the flavonoid content of EGb and eluent volume. To keep the quality, processing condi- tions and yield of EGb stable, it was suggested to mix G. biloba leaves with different flavonoid concentration to make the flavonoid content in the leaves be 1.0%. By using twice amount of 15% ethanol to elute the resins, EGb containing more than 24% of flavonoids and 6% of lactone can be prepared with yield of 2.6% and over 66% flavonoid extraction rate.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2017年第12期2097-2103,2091,共8页
Natural Product Research and Development
关键词
银杏叶提取物
银杏叶质量
黄酮醇苷
Ginkgo biloba extract
Ginkgo leaves
flavonoid glycosides