秦艽环烯醚萜苷类成分分离纯化工艺及抑菌活性研究

Separation,Purification and Antibacterial Activity of Iridoid glycosides from Gentiana macrophylla Pall.

研究优化XDA-1大孔吸附树脂分离纯化秦艽环烯醚萜苷的最佳工艺,并测试其纯化产物的抑菌活性。以龙胆苦苷的含量为指标,利用动态吸附分离方法,确定秦艽环烯醚萜苷的最佳分离纯化工艺。结果表明XDA-1大孔吸附树脂分离纯化秦艽环烯醚萜苷类化学成分的最佳工艺条件为:上样液浓度0.07 g原药材/mL,pH值为5.0,吸附流速4 BV/h,上样液体积32 BV,洗脱剂浓度50%乙醇溶液,pH值7.0,解吸附流速3 BV/h,洗脱剂用量为8 BV,纯化后环烯醚萜苷含量可达62.97%,并证明了所选的大孔树脂纯化工艺稳定、可靠,值得在生产中推广应用。通过对3种细菌抑菌圈和最小抑菌浓度测定,初步评判该纯化产物的抑菌活性,结果表明,该纯化后产物具有一定的抑菌活性。

The aim of this study was to optimize the separation and purification technology of iridoid glycosides from Gen- tiana macrophylla Pall. with XDA-1 macroporous adsorption resin. The antibacterial activity of the purified iridoid glyco- sides were studied in vitro. With the content of gentiopicroside as index, dynamic adsorption-separation was used to deter- mine the optimal separation and purification conditions of iridoid glycoside from G. macrophylla. The optimal conditions of XDA-1 macroporous adsorption resin were finally determined as follows ： sample injection concentration was 0.07 g o- riginal/mL,pH was 5. O, sample flow rate was 4 BV/h, quantity of adsorption was 32 BV, desorption rate was 3 BV/h, the pH was 7.0 and elution volume was 10 BV of 50% ethanol. The purity of obtained iridoid glycosides was 62.97%. Moreover,the antibacterial activity of the extract was evaluated by 3 bacteria inhibition zone and minimum inhibitory concentration. The results showed that the obtained iridoid glycosides had good antibacterial activity.