祛痰通阳汤对慢性心力衰竭模型大鼠超声心动图及血清AngⅡ含量、心肌组织AT1 mRNA表达的影响

Effect of Qutan Tongyang Decoction (祛痰通阳汤) on Echocardiography,Serum Ang Ⅱ Level and Expression of AT1 mRNA in Myocardium of Chronic Heart Failure Model Rats

目的探讨祛痰通阳汤治疗慢性心力衰竭的可能作用机制。方法将90只雄性Wistar大鼠随机分为正常组、模型组、卡托普利组和中药低、中、高剂量组,每组15只。除正常组外,其余各组均给予注射用盐酸多柔比星阿霉素腹腔注射制备慢性心力衰竭大鼠模型。造模后卡托普利组给予卡托普利片4.375 mg/(kg·d)灌胃,中药低、中、高剂量组每日分别给予祛痰通阳汤水煎液(浓度1.85 mg/ml)1.8、3.6、7.2 ml灌胃,正常组和模型组给予3.6ml蒸馏水灌胃,每天灌胃1次,连续4周。测定各组大鼠血清血管紧张素Ⅱ(AngⅡ)含量及心肌组织中血管紧张素Ⅱ1型受体(AT1)mRNA水平,造模前、造模后及给药后检测各组大鼠超声心动图参数[包括左心室舒张末期内径(LVEDD)、每搏心输出量(SV)和射血分数(EF)]变化情况。结果与正常组比较,模型组大鼠血清AngⅡ、心肌组织AT1 mRNA水平、LVEDD显著增加,SV、EF降低(P<0.01)。与模型组比较,治疗后卡托普利组和中药中、高剂量组大鼠血清AngⅡ含量、心肌组织AT1 mRNA水平降低,LVEDD降低,SV和EF均增加(P<0.05或P<0.01)。结论祛痰通阳汤可降低慢性心力衰竭大鼠血清中AngⅡ含量,抑制AT1 mRNA表达,改善心功能,从而改善心肌重构。

Objective To explore the possible mechanism of Qutan Tongyang Decoction（ 祛痰通阳汤） in the treatment of chronic heart failure. Methods Ninety male Wistar rats were randomly divided into normal group,model group,captopril group,and low,middle and high dose Chinese medicine groups,with 15 rats in each group.Except for normal group,rats were given intraperitoneal injection of doxorubicin hydrochloride injection to prepare the chronic heart failure rat model. After modeling,captopril group was given captopril tablets 4. 375 mg/（ kg · d） by gavage. Low,middle and high dose Chinese medicine groups were respectively given 1. 8,3. 6 and 7. 2 ml Qutan Tongyang Decoction（ concentration 1. 85 mg/ml） by gavage each day. Normal group and model group were given 3. 6 ml distilled water by gavage,once a day and lasted for 4 weeks. The levels of serum Ang Ⅱ and AT1 mRNA in myocardium in each group were determined. Echocardiography parameters including left ventricular end diastolic diameter（ LVEDD）,stroke volume（ SV） and ejection fraction（ EF） were detected before and after modeling and after administration. Results Compared with normal group,the level of serum Ang Ⅱ and myocardial AT1 mRNA and LVEDD were significantly increased in the model group and the SV and EF were decreased in the model group（ P 0. 01）. Compared with the model group,the levels of serum Ang Ⅱ and myocardial AT1 mRNA and LVEDD in captopril group and middle and high dose Chinese medicine groups were decreased and SV and EF in captopril group and middle and high dose Chinese medicine group were both increased（ P 0. 05 or P 0. 01）. Conclusion Qutan Tongyang Decoction could reduce the level of serum Ang Ⅱ in the rats with chronic heart failure,inhibit the expression of AT1 mRNA and improve the cardiac function so that the myocardial remodeling could be improved.