人HNRNPA1基因及蛋白质的生物信息学分析

Bioinformatic analysis of HNRNPA1 gene and protein in human

采用生物信息学的方法分析人HNRNPA1基因的启动子情况及蛋白质的理化性质、信号肽及NLS、亲疏水性、跨膜区域、蛋白质结构、相互作用的蛋白质及GO注释.选择合适软件对HNRNPA1相关信息进行分析.结果显示,预测的HNRNPA1基因存在1个启动子;HNRNPA1蛋白质是由372个氨基酸组成的具有NLS、无跨膜结构的亲水不稳定蛋白质,其等电点为9.17;哺乳动物中氨基酸序列高度保守;二级结构以无规卷曲为主,预测的三级结构经拉曼图分析可信度高;HNRNPA1多定位于细胞核,与RNA的选择性剪接及mRNA运输有关.此外,启动子的甲基化对HNRNPA1表达影响明显,其蛋白质具有NLS、无跨膜结构且不稳定,属于亲水蛋白质,分布于细胞核,对mRNA的成熟具有重要作用.

To analyze the promoter, physicochemical property, signal peptide, NLS, transmembrane domain, hydrophilcity/hydrophobieity, secondary structure, teriary structure, protein-protein interaction and Gene Ontology of HNRNPA1, bioinformation and multiple analysis software were used. The results showed that there was 1 promoters in HNRNPA1. And HNRNPA1 protein was composed of 372 amino acids, with NLS and without transmembrance domain with isoelectric point of 9.17, and it was highly conservative in mammal. There were some random coils in the secondary structure predicted. The 3 D model predicted was credible by Ramachandran plot analysis. HNRNPA1 located in nucleus, and took part in RNA alternative splicing and mRNA transportation. HNRNPA1 was a hydrophilic and unstable protein with NLS and without transmembrance domain. HNRNPA1 regulated RNA alternative splicing and mRNA transportation.