炎症反应中组织损伤机制的探讨:中性粒细胞化学发光和脱颗粒的研究

Stimulated Chemiluminescence and Degranulation of Human Polymorphonuclear Leukocytes: Possible Involvement in the Mechanisms of Tissue Damage During Inflammation

以大鼠脱精氨酸C5a(r-C5ai)、人工合成趋化因子N-甲酰-甲硫氨酰一亮氨酰-苯丙氨酸(fMet-Leu-phe,简称FMLP),血清调理的酵母多糖(serum treated zymosan,简称STZ)、热聚IgG(A-IgG)、钙离子载体A23187为刺激物,观察它们对中性粒细胞(PMN)化学发光和脱颗粒的影响,以便为以炎症反应为特征的疾病的发病学研究提供线索。结果表明,所用刺激物均显著提高PMN的化学发光,但不同刺激物对PMN脱颗粒时不同酶的释放影响不同。

A significant increase of polymorphonuclear leukocytes (PMN) chemilum-inescence (CL) was observed when PMN was treated with rat C5ades Arg (r-C5ai), FMLP, opsozined zymosan (STZ) or a calcium ionophore A23487 separately. These stimuli, as well as aggregated IgG (A-IgG), could also cause the release of β-glucuronidase (β-g) and myeloperoxidase (MPO) from PMN. on the Other band, elastase (NE) release was pot noticed when PMN was trea.- ted with r-C5ai and FMLP, which generally stimulated PMN in a cytochalasin B-dependent manner. These results suggest that the kinetics of PMN CL and degranulation vary depending upon the stimulus.