消退素D1治疗实验性大鼠牙周炎的研究

Curative Effect of Endogenous Resolvin D1 on Periodontitis in Rats

目的:探讨消退素D1(RvD1)对治疗牙周炎的作用。方法:取18只大鼠建立牙周炎模型,按随机化原则均分为实验甲组(100μg/L的RvD1),实验乙组(50μg/L的RvD1)及阴性对照丙组(生理盐水)。所有大鼠均经尾静脉注射给药进行治疗。治疗后不同时间进行临床检查和形态学测量。采用SPSS21.0软件包对数据进行单因素方差分析。结果:治疗后,实验甲组(100μg/L RvD1)中牙周袋深度、牙龈指数、松动度、牙槽骨丧失量均低于阴性对照组(P<0.05)。实验乙组(50μg/L RvD1)的以上4个指标虽均低于阴性对照组,但仅牙龈指数和松动度与阴性对照组的差异有统计学意义(P<0.05)。两实验组治疗后的牙周袋深度相近(P>0.05)。结论:两种浓度的RvD1对实验性大鼠牙周炎有治疗作用;100μg/L的RvD1可阻止实验性大鼠牙周炎的牙槽骨继续破坏。

Objective： To investigate the effect of resolvin D1 （RvD1） on periodontitis in rat. Methods： The models of periodontitis were established in eighteen rats, which were randomly divided into 3 groups, experimental group A （100ng/ml RvD1）, experimental group B （50ng/ml RvD1）, and control group C （saline）. Intervention was provid ed by systemic injection from the tail vein. Clinical parameters and morphological analysis were measured at differ- ent time intervals after treatment. All statistical analysis was performed using the SPSS 21.0 statistical package. Results： After 1 week of treatment, periodontal pocket depth （PD）, gingival index （GI）, tooth mobility （TM）, and alveolar bone loss （ABL） in experimental group A （100ng/ml RvD1） were lower than those in the control group （P d0.05）. For experimental group B （50ng/ml RvD1）, only GI and TM showed statistical significance when corn pared with control group （P（0.05）. PD in both experimental groups was similar. Conclusion： RvD1 might be used to prevent alveolar bone resorption in periodontitis.