摘要
The induction, subculture and differentiation of callus from immature embryos of maize （ Zea mays L. ） inbred line were studied and optimized. The re- suits revealed that, 2 mg/L 2,4-D was the optimal concentration to induce embryonic callus. Calli induced from inbred Qi319 and LY92 had good morphology and high regeneration. The embryos of the two inbred lines were selected as explants to establish efficient and stable tissue culture and transformation system.
The induction, subculture and differentiation of callus from immature embryos of maize （ Zea mays L. ） inbred line were studied and optimized. The re- suits revealed that, 2 mg/L 2,4-D was the optimal concentration to induce embryonic callus. Calli induced from inbred Qi319 and LY92 had good morphology and high regeneration. The embryos of the two inbred lines were selected as explants to establish efficient and stable tissue culture and transformation system.
基金
Supported by Science and Technology Development Plan of Shandong Province
National Key Laboratory Open Project of Crop Biology(2014KF11)
