金纳米颗粒表面能量转移及巯基化合物的检测

Gold Nanopaticle Surface Energy Transfer and Its Application for Thiols Detection

二氧化硅稳定的金纳米颗粒(Au-Si O_2)与罗丹明B之间发生表面能量转移,使罗丹明B荧光猝灭。金纳米颗粒对罗丹明B的Stern-Volmer猝灭常数为4.3×103L/mol。当荧光猝灭的混合体系中加入巯基化合物时,巯基化合物与金纳米颗粒发生强相互作用阻断罗丹明B-金纳米颗粒之间的能量转移,罗丹明B荧光恢复。基于罗丹明B-Au-Si O_2体系对巯基化合物的单一响应,建立了一种简单快速检测巯基化合物的方法;并且由于二氧化硅对金纳米颗粒的稳定作用,金纳米颗粒成为一种可以回收利用的检测探针。

The fluorescence of Rhodamine B was quenched due to the surface energy transfer from Rhodamine B to silica supported gold nanoparticle surface. The Stem-Volmer quenching constant of the gold nanoparticles was 4. 3 × 103 L/mol in this system. While, the fluorescence of Rhodamine B recovered after the addition of thiols due to the strong affinity of thiol to gold nanoparticles which obstructed the energy transfer between Rhodamine B and gold nanoparticles. As the unique response of Rhodamine B-Au-SiO2 system to thiols, it provides a simple analytical method for the detection of thiols. Moreover, Au-SiO2 becomes a recyclable probe because of the stabilization of silica support.