免标记光学生物传感器研究进展

Advances in Label-Free Optical Biosensor

免标记光学生物传感器因分析样品无需标记、强特异性、动态测量、无损检测、分析速度快等诸多优点,在化学、药学及生物等诸多领域中得到了快速发展和广泛应用。本文重点介绍了当前发展成熟且具代表性的四种免标记光学生物传感器,即表面等离子体共振传感器、光波导光模光谱传感器、椭圆偏振光学传感器以及反射干涉传感器,对每种传感器的原理结构、方法发展及在生化分析等相关领域中的最新应用进行了总结和论述;在此基础上,对它们各自的性能进行了优劣比较;最后对免标记光学生物传感器的发展前景进行了展望。

Label-free optical biosensor has been widely studied and applied to the research fields including chemistry, pharmacy and biology associated with life science recently, due to its key features such as label-free of target molecule, dynamic monitoring, non-destructive detection and so on. This article mainly focuses on the current development of four representative kinds of label-free optical biosensors, including surface plasmon resonance biosensor, optical waveguide light mode spectroscopy biosensor, spectroscopic ellipsometry biosensor, and reflectometry interference spectroscopy biosensor. In addition to the introduction of the instrument structure and mechanism, method development, and applications in various fields, the advantages and shortcomings of these methods are discussed, which may illuminate the future development.