影响α_2-巨球蛋白水溶液热稳定性的多因素分析

Analysis of Factors Affecting the Thermal Stability of α2-Macroglobulin in Solution

用正交试验法对影响α_2-巨球蛋白水溶液热稳定性的多种因素进行了分析,确定对α_2-巨球蛋白加热处理的最佳条件。在该条件下,α_2-巨球蛋白经60℃10h加热处理后可保留全部结合胰蛋白酶活性。

The factors affecting the thermal stability of α2-macroglobulin (α2M) in aqueous solution have been analyzed with an experiment of orthogonal design. The optimum conditions for heat treatment of α2M have been established. Under these conditions.the trypsin-binding activity of α2M can be fully retained after heat treatment at 60℃ for 10 hours.