花椒麻味物质分子印迹固相萃取柱的制备及其应用

The preparation and application of the Chinese prickly ash's molecularly imprinted solid-phase extraction

以α-(羟基)-山椒素分子结构类似物为模板分子、2-乙烯基吡啶(2-vinylpyridine,2-Vpy)为功能单体、二甲基丙烯酸乙二醇酯(ethyleneglycol dimethacrylate,EDMA)为交联剂,按1∶4∶20的摩尔比合成了花椒麻味物质硅胶表面分子印迹聚合物,并以合成的硅胶表面分子印迹聚合物为固相萃取柱填料,制备了花椒麻味物质分子印迹固相萃取柱,确定了该固相萃取柱的最佳上样溶剂、淋洗溶剂及洗脱溶剂,用此固相萃取柱对花椒油树脂中的花椒麻味物质进行分离纯化。结果表明,该固相萃取柱对花椒麻味物质表现出优异的特异性吸附效果,可以分离纯化出相对纯度为93.66%的花椒麻味物质。

The molecularly imprinted polymers was synthesized by molecular structure analogues of α-（ OH）-sanshool as the template molecule,2-Vpy as functional monomer,EDMA as the crosslinking agent and at the proportion of 1∶ 4∶ 20. The optimal sample solvent,drip washing solvent and elution solvent were selected out and the SMIP was used to separate and purify the numb taste components in Zanthoxylum oleoresin. The results show that the SMIP had specific adsorption to the numb taste components,and 93. 66% of pure numb taste components can be separated.