草苁蓉环烯醚萜苷含药血清诱导人肝癌SMMC-7721细胞凋亡中PI3K/Akt通路的作用

The effect of PI3K/Akt pathway in the apoptosis of human hepatoma MMC-7721 cells induced by serum containing Iridoid glycosides from Boschniakia rossica

研究草苁蓉环烯醚萜苷(IGBR)含药血清对人肝癌SMMC-7721细胞活力与凋亡的影响并探讨其可能作用机制。MTT法检测IGBR含药血清对人肝癌SMMC-7721细胞活力的影响,流式细胞仪检测细胞凋亡,Western blot检测PI3K/Akt信号通路相关蛋白。结果表明,IGBR含药血清可降低人肝癌SMMC-7721细胞活力,诱导细胞凋亡。与对照组比较,5-FU组与IGBR组人肝癌SMMC-7721细胞中Bax蛋白表达增多,而p-Akt和Bcl-2蛋白的表达减少(p<0.05),两组间无明显差异(p>0.05);而总Akt没有明显改变。IGBR含药血清可通过PI3K/Akt信号通路,调节Bcl-2家族成员(Bax和Bcl-2),诱发人肝癌SMMC-7721细胞凋亡。

The aim of this study was to investigate the effect of Iridoid glycosides from Boschniakia rossica （IGBR） serum on the viability and apoptosis of human hepatoma SMMC-7721 cells, and to explore the possible mechanism.MTr method was used to detect the effect of IGBR containing serum on the viability of human hepatoma SMMC-7721 cells.Apoptosis was detected by flow cytometry, and Western blot was used to detect PI3K/Akt pathway related proteins. The results indicated that IGBR containing serum could reduce the viability of SMMC-7721 hepatoma cells and induce apoptosis. Fluorescence microscopy showed that apoptotic cells were enhanced by staining, and the fluorescence was bright, showing a uniform round and mass like structure.Western blot results compared with control group,5-FU group and IGBR SMMC-7721 cells in the expression of Bax prtein increased, and p-Akt, reduced the Bcl-2 protein expression （p 〈 0.05 ）, no obvious difference between the two groups （p 〉 0.05） ,while the total Akt did not change significantly.The IGBR containing serum can regulate Bax and Bcl-2 through PI3K/Akt signaling pathway and induce apoptosis of human hepatoma SMMC-772! cells.