红树林植物阔苞菊内生真菌硒富集工艺优化

Optimized process of selenium enrichment for endophytic fungi from mangrove plant Pluchea indica Less.

为了获得有机硒的来源新途径,以菌丝体的富硒总量为指标,采用单因素、正交实验法研究了一株阔苞菊内生真菌的硒富集能力,对培养基中硒溶液添加量、盐浓度、碳源浓度、培养时间、碳源种类等影响因素进行了优化。结果表明,适合的培养条件为硒溶液添加量为30 m L、盐浓度5 g/L、碳源(葡萄糖)浓度15 g/L、培养时间24 h;代谢产物中硒含量为181.6μg/g,菌丝体富硒总量为1941.7μg/L,其有机硒转化率偏低,仅为16.2%。为进一步筛选开发滨海红树林中内生富硒微生物资源提供理论依据。

Study of the selenium enrichment ability for an mangrove plant Pluchea indica endophytic fungus by means of single factor and orthogonal experiments,in order to obtain organic selenium.The influence factors such as selenium solution added amount, salt concentration, carbon source concentration, culture time and carbon source were optimized with the total selenium accumulation as the index in the experiment. The results showed that when the enriched culture medium was contained salt concentration 5 g/L, selenium solution added volmne 30 mL,carbon source（glucose） concentration 15 g/L, culture time 24 h, the total selenium accumulation was 1941.7 g/L,the mycelium Se content of up to 181.6 μg/g and the lower organic selenium transformation rate was 16.2%. It is expected to provide theoretical basis for further researching selenium rich microbial resources in coastal mangrove plants.