响应面法优化龙胆草多糖的脱色工艺

Optimization of decolorization of polysaccharide from gentian by response surface methodology

采用响应面法优化聚酰胺对龙胆草多糖的脱色工艺。在单因素实验的基础上,选取龙胆草多糖脱色过程中的上样体积、上样流速和上样浓度为影响因素,以龙胆草多糖的脱色率和多糖保留率的综合评分(OD)为响应值,由Box-Behnken实验设计对龙胆草多糖的脱色工艺进行响应面分析,对龙胆草多糖的脱色工艺进行优化。结果表明,最佳脱色条件为:上样体积为5 BV,上样流速为2 BV/h,上样浓度为3.50 mg/m L。在此条件下,龙胆草多糖的脱色率为80.60%±0.91%,多糖保留率为86.28%±0.67%,其综合评分(OD)为0.8344,而模型预测的OD值为0.8756,两者误差较小。聚酰胺的重复利用实验表明,经过5次重复使用后,脱色率降为76.85%±0.59%,多糖保留率有所增大到98.32%±0.37%,循环使用效果较好,该优化工艺可靠。

The purpose was to optimize the decolorization process of polyamide for gentian polysaccharide by response surface methodology.On the basis of single factor test, sample volume, flow rate and the concentration of polysaccharide from gentian were selected as influential factors.The comprehensive score（OD） including the decolorization rate and polysaccharide retention rate was used as response value. The response surface analysis was carded out by Box-Behnken to optimize the process of decolorization process of gentian polysaccharide.The results indicated that the optimal decoloration process conditions were as follows：the sample size was 5BV,the sample flow rate was 2BV/h, and the sample concentration was 3.50 mg/mL.Under these conditions,the decolorization rate of polysaccharide was 80.60% ± 0.91% , and the retention rate of polysaccharide was about 86.28% ± 0.67%.The predicted values of the model was 0.8756 and the score（OD） was about 0.8344, which showed a small error between both of them.After 5 times of reuse,the decolorization rate decreased to 76.85% ± 0.30% and the retention rate of polysaccharide increased to 98.32% ±0.37%.So the polyamide has a good recycling effect, and the optimization process is reliable.