五味子糖蛋白的纯化及其抗氧化活性

Purification and antioxidant activity of glycoprotein from Schisandra chinensis

本文研究了五味子糖蛋白Sevage法除游离蛋白的最佳工艺条件,采用碱提酸沉法提取得到五味子糖蛋白,以蛋白质清除率和多糖损失率作为考察指标,分别考察氯仿与正丁醇的比例、样液与Sevage试剂的比例、振摇时间、脱蛋白次数对除蛋白效果的影响,在单因素实验的基础上采用正交及响应面实验优化工艺条件,得到响应面优化后结果最佳,即氯仿与正丁醇的比例为3∶1,样液与Sevage试剂的比例为3∶1,振摇时间为14 min,脱蛋白次数为3次,此条件下的蛋白质平均清除率为30.09%,多糖平均损失率为4.71%,综合评分平均值为88.11。体外抗氧化活性结果表明,五味子糖蛋白对于DPPH自由基和超氧阴离子清除能力较强(IC50为1.08 mg/m L和0.80 mg/m L),并具有一定的还原能力。研究表明响应面法优选工艺简便高效;五味子糖蛋白具有体外抗氧化活性,值得进一步研究。

In this paper,the optimum conditions of Schisandra chinensis glycoprotein Sevage method were studied.Glycoprotein of Schisandra chinensis was obtained from alkali extraction and acid precipitation method. Protein clearance rate and polysaceharide loss rate were used as the index of investigation, the effects of the ratio of chloroform and n - butanol, the proportion of sample solution and Sevage reagent, the shaking time and the number of proteins on the removal of protein removal were investigated.On the basis of single factor test, orthogonal design and response surface test were used to optimize the process conditions, the results showed that the ratio of chloroform to n-butanol was 3： 1, the ratio of sample to Sevage was 3： 1, shaking time of 14 minutes,deproteinization times 3 times,under these conditions, the average clearance rate of protein was 30.09% , and the average loss rate of polysaccharide was 4.71% , the average score for the comprehensive score was 88.11.In vitro antioxidant activity showed that Schisandra chinensis glyeoprotein had strong ability to scavenge DPPH free radical and superoxide anion（IC50 1.08 mg/mL and 0.80 mg/mL） ,and had some reduction ability.The results showed that the response surface method was simple and efficient, and the Schisandra chinensis glycoprotein had its antioxidant activity in vitro and deserved further study.