豆浆中挥发性成分顶空固相微萃取条件的优化

Optimization of headspace solid phase microextraction conditions for GC-MS analysis of volatile components in soymilk

采用顶空固相微萃取(HS-SPME)与气质联用技术(GC-MS)测定豆浆的挥发性成分,对影响顶空固相微萃取的关键因素盐离子浓度、搅拌速度、萃取温度、萃取时间和解析时间进行分析,得到最佳提取条件。结果表明:顶空固相微萃取豆浆挥发性成分最优条件为5 m L豆浆中加入0.5 g Na Cl,使用DVB/CAR/PDMS-50/30μm萃取头,搅拌速度600 r/min,搅拌的同时进行吸附作用,萃取温度40℃条件下顶空吸附30 min,然后在250℃的GC进样口解析9 min。在该萃取条件下,利用顶空固相微萃取能够从研究用的豆浆中萃取到23种挥发性成分,醛类10种、醇类5种、酮类3种、呋喃2种,酯类1种、其他类2种,占峰面积总和的90.48%。

The volatile components of soybean milk were analyzed by Headspace solid phase microextraction （ HS- SPME ） coupled with gas chromatography- mass spectrometry （GC- MS）. The key factors affecting the headspaee solid phase microextraction the effects of salt concentration, stirring rate, extraction temperature, extraction time, and desorption time on the extraction efficiency were analyzed to get the optimum extration conditon.The results showed that the optimum conditions of volatile components in soybean milk by headspace solid phase microextraction were adding the 0.5 g NaCI to 5 mL soymilk, using DVB/CAR/PDMS-50/30 μm extraction fiber, adsorbing while stirring at the speed of 600 r/rain, stirring and absorbing simultaneously,absorbing 30 rain under the condition of the extraction temperature of 40 ℃, and then desorbing 9 min at the GC inlet at 250 ℃.Under the conditions of extraction,23 kinds of volatile components were extracted from soybean milk by using headspacc solid phase microextraction,accounting for 90.48% of the total peak area.There were l0 kinds of aldehydes,5 kinds of alcohols,3 kinds of ketones,2 kinds of furan, 1 kinds of esters, and other 2 kinds.