毛细管电泳电化学发光法快速分离检测水果中的精胺

Rapid separation and determination of spermine in fruit by capillary electrophoresis with electrochemiluminescence detection

目的:建立水果中精胺含量测定的毛细管电泳电化学发光(CE-ECL)法。方法:利用CE-ECL技术建立了一种高效快速准确的测定桑椹、苹果、草莓中精胺含量的的方法。结果:精胺标准溶液在2×10^(-4)~3×10^(-1)mmol/L浓度范围内与ECL强度呈良好的线性关系,其线性方程为I=1114.2c+99.41,相关系数r=0.9996(n=5),检出限(S/N=3)为9.9×10^(-5)mmol/L,加标回收率分别为95.14%~101.15%、98.97%~100.82%和100.57%~106.11%。结论:该方法简单、准确,重复性好,可用于研究果蔬采后保鲜贮藏和延长货架期。

Objective： To establish a capillary electrophoresis with electrochemiluminescence （CE- ECL ）method for determination of spermidine in fruit. Methods： An efficient, rapid and accurate analytical approach was developed for the determination of spermine in mulberry, apple, strawberry by CE-ECL technique.Results：The ECL intensity was linear with the concentrations of spermine over the ranges from 2 ×10^-4-3×10 ^-1 mmol/L.The linear equation was I = 1114.2c ＋ 99.41, correlation coefficient r = 0.9996 （ n = 5 ） with detection limit （ S/N = 3 ） of 9.9 × 10^-5 mmol/L.The adding standard recoveries were 95.14% - 101.15% , 98.97% - 100.82% and 100.57% - 106.11% , respectively. Conclusions： This method is simple, accurate, reproducible and can be used for the study of fruit fresh-keeping, and prolonging processing period.