摘要
目的:建立水果中精胺含量测定的毛细管电泳电化学发光(CE-ECL)法。方法:利用CE-ECL技术建立了一种高效快速准确的测定桑椹、苹果、草莓中精胺含量的的方法。结果:精胺标准溶液在2×10^(-4)~3×10^(-1)mmol/L浓度范围内与ECL强度呈良好的线性关系,其线性方程为I=1114.2c+99.41,相关系数r=0.9996(n=5),检出限(S/N=3)为9.9×10^(-5)mmol/L,加标回收率分别为95.14%~101.15%、98.97%~100.82%和100.57%~106.11%。结论:该方法简单、准确,重复性好,可用于研究果蔬采后保鲜贮藏和延长货架期。
Objective: To establish a capillary electrophoresis with electrochemiluminescence (CE- ECL )method for determination of spermidine in fruit. Methods: An efficient, rapid and accurate analytical approach was developed for the determination of spermine in mulberry, apple, strawberry by CE-ECL technique.Results:The ECL intensity was linear with the concentrations of spermine over the ranges from 2 ×10^-4-3×10 ^-1 mmol/L.The linear equation was I = 1114.2c + 99.41, correlation coefficient r = 0.9996 ( n = 5 ) with detection limit ( S/N = 3 ) of 9.9 × 10^-5 mmol/L.The adding standard recoveries were 95.14% - 101.15% , 98.97% - 100.82% and 100.57% - 106.11% , respectively. Conclusions: This method is simple, accurate, reproducible and can be used for the study of fruit fresh-keeping, and prolonging processing period.
出处
《食品工业科技》
CAS
CSCD
北大核心
2018年第1期267-271,共5页
Science and Technology of Food Industry
基金
陕西省教育厅2017年重点科学研究计划(17JS024)
陕西理工大学博士后专项项目(SLGBH16-01)
关键词
毛细管电泳
电化学发光
钌联吡啶
精胺
capillary electrophoresls
electrochemllummescence
Ru (pby) 3^2+
spermine