鱼腥蓝细菌PCC 7120中可控降解系统的构建

Construction of controllable protein degradation system in Anabaena sp. PCC 7120

鱼腥蓝细菌PCC 7120中已有较为成熟的诱导表达系统,但缺乏可控的蛋白降解系统。本研究基于Mesoplasma florum中的Lon蛋白酶(mf-Lon),在蓝细菌中建立可诱导的蛋白降解系统,并通过该系统对关键基因编码产物进行可控降解以研究其生理功能。结果发现降解系统并无预期作用,需进一步改进。

Anabaena sp. PCC 7120 is an important model strain in the field of eyanobacteria re-search.Controlling the expression level of gene is one of the most important ways to investigate gene functions.There are a variety of matured gene induction systems available in cyanobacteria. However,no controllable protein degradation system is available in cyanobacteria yet. This work is based on the pro- tein quality control system in Mesoplasrna florum and aims to construct an inducible protein degradation system facilitating the investigation of essential genes in Anabaena PCC 7120. Results showed that the degradation or decrease of the target proteins was not detected.It is indicated that the constructed system needs to be further improved.