某种鸡群沙门菌的分离、鉴定及毒力基因检测

Isolation,identification and the detection of virulence genes of Salmonella isolated from a breeder flock

为了解河南省某父母代种鸡场死胚中沙门菌的携带情况及毒力基因分布特征,采集孵化后期死胚120份,通过常规分离、PCR鉴定及血清学鉴定,并采用PCR方法对分离菌株进行10种毒力基因检测。结果:获得了55株沙门菌,包括6种血清型:鸡白痢沙门菌(25)、爱丁堡沙门菌(17)、汤卜逊沙门菌(10)、塞罗沙门菌(1)、加瓦尼沙门菌(1)、坦密尔纳德沙门菌(1);毒力基因sopB、usherP检出率为100%,毒力基因spvC(34.55%)、sodC1(45.45%)检出率较低。结果表明:该种鸡场感染的沙门菌血清型复杂,毒力基因普遍存在于沙门菌分离株中。

To investigate the prevalence and the distribution characteristics of virulence genes of Salmonella obtained from the parent chicken farm in Henan province,Salmonella were isolated from the collected 120 dead embryos by the conventional isolation,PCR identification,serological? identification,and the isolates were tested for 10 virulence genes by PCR method.Results: 55 Salmonella isolates were obtained,including six serotypes: S. pullorum( 25),S. Edinburg( 17),S. Thompson( 10),S. Cerro( 1),S. gatuni( 1),S. Tamilnadu( 1). The positive rate of virulence gene sop B,usher P were both 100%,and that of spvC( 34. 55%),sodC1( 45. 45%) was lower. The results indicated that the Salmonella serotypes was complex in the chicken stock,and the virulence genes were widely existed in Salmonella isolates.