大豆抗菌核病位点挖掘及一致性QTL分析

Identification of QTL and consistency QTL with resistance to Sclerotinia sclerotiorum in soybean

利用离体叶柄接种法和活体茎接种法对以感病大豆品种合丰25和抗病大豆品种Maple Arrow为亲本构建的128份后代群体进行鉴定,通过复合区间作图法结合表型鉴定数据定位到7个抗大豆菌核病相关的QTL位点。收集整理在B1连锁群上关于大豆抗病虫害的QTL位点,通过元分析构建大豆抗病虫害相关的一致性QTL图谱,并得到了2个一致的QTL,缩小了置信区间并发掘了21个候选基因,其中3个与抗病相关。本研究得到的QTL在B1连锁群上定位的QTL位点附近,这为抗大豆菌核病精细定位和基因克隆奠定了基础。

A total of 128 lines derived from a cross between a susceptible soybean cultivar Hefeng 25 and a disease resistant soybean cultivar Maple Arrow,were analyzed through isolated leaf stalk inoculation method and living stem inoculation method. A total of 7 QTLs were detected through combining 2 sets of phenotypic identification data with the interval mapping method. QTL for the soybean with pests or disease-resistance was collected through meta-analysis,the consistent QTL map for the soybean with pests or disease-resistance was constructed,and 2 consistent QTL were obtained. The more reduced confidence interval was obtained and 21 candidate genes were discovered,3 of which were associated with disease resistance. The consistent results were similar to that of this study in B1 linkage group,which provided a research basis for fine positioning and gene cloning.