新型营养沙棘醋制备工艺研究

Study on the preparation of new nutrition seabuckthorn vinegar

旨在开发一种营养型沙棘果醋的理想制备工艺.以南疆优质沙棘果为原料,新疆食醋为溶剂,通过超声辅助技术对沙棘功能成分-黄酮类化合物、维生素C和超氧化物歧化酶进行浸提,考察了食醋浓度、液固比、超声温度、超声功率、超声时间和浸提次数对总黄酮得率、维生素C(VC)得率和超氧化物歧化酶(SOD)活力的影响,并对浸提工艺条件进行了响应面法优化;对沙棘汁醋酸发酵过程进行了研究,将发酵沙棘醋与浸提沙棘醋进行了对比.结果表明,食醋浸提工艺适宜条件为:食醋浓度9.4g/L、液固比30∶1mL/g、超声时间40min、超声温度55℃、超声功率80w、浸提次数3次,在此条件下,浸提沙棘醋里的总黄酮、VC和SOD含量分别为35.98mg/100mL、17.85mg/100mL和121.29U/mL;而发酵沙棘醋相对较低,分别为14.72mg/100mL、5.31mg/100mL和53.99U/mL.因此,新疆食醋浸提南疆沙棘果是营养沙棘醋的理想工艺,其过程简单、易操作.

The purpose of this study is to develop an ideal preparation process for nutritious seabuckthornfruit vinegar.To attain this aim,the high-quality seabuckthornfruit and vinegar from southern Xinjiang were used as raw materials to extract flavonoids,vitamin C(VC)and superoxide dismutase by ultrasonic assisted technique.The effects of the vinegar concentration,the ratio of liquid to solid,ultrasonic temperature,ultrasonic power,ultrasonic time and the extraction times on the total flavonoid yield,vitamin C yield and superoxide dismutase(SOD)activity were investigated using single-factor tests.And the optimal extraction conditions were determined by a response surface methodology.Seabuckthorn juice was also used to ferment seabuckthorn vinegar and the comparative study was conducted between the extracted seabuckthorn vinegar and the fermented seabuckthorn vinegar.The results showed that the optimal extraction conditions were vinegar concentration of 9.4 g/L,liquid to solid ratio of 30∶1 mL/g,ultrasonic time of 40 min,ultrasonic temperature of 55 ℃,ultrasonic power of 80 wand extraction times of 3 times.Under these conditions,the contents of total flavonoid,VCand SOD in extracted seabuckthorn vinegar were 35.98 mg/100 mL,17.85 mg/100 mL and 121.29 U/mL respectively,while they were relatively low in fermented seabuckthorn vinegar,corresponding to 14.72 mg/100 mL,5.31 mg/100 mL and 53.99 U/mL.It is concluded that the extraction method is an ideal way for the production of healthy southern Xinjiang seabuckthorn fruit vinegar compared with the fermentation method,which is simple,easy to operate.