肉苁蓉提取物对H_2O_2致兔肾小管上皮细胞损伤的影响

Effect of Cistanches deserticola Extract on Injury of Rabbit RTECs by H_2O_2

通过体外培养兔肾小管上皮细胞,研究H_2O_2对细胞增殖作用及肉苁蓉提取物对H_2O_2致肾小管上皮细胞损伤的影响。通过细胞形态观察,采用MTT法测定H_2O_2对兔肾小管上皮细胞增殖作用的影响及肉苁蓉水提、醇提物2、20、200mg·L-1三个剂量组的保护作用,测定细胞LDH释放量。结果表明,随着H_2O_2浓度的增加,形态改变的细胞数量逐渐增多,贴壁细胞数量逐渐减少,细胞增殖抑制率越大,细胞LDH释放量越多,呈浓度依赖性,肉苁蓉醇提物和水提物高浓度组能够显著降低H_2O_2对兔肾小管上皮细胞增殖的抑制作用(P<0.01),肉苁蓉醇提物和水提物的高、中、低剂量组均能使H_2O_2作用后兔肾小管上皮细胞LDH释放量极显著低于模型组LDH的释放量(P<0.01),结果呈浓度依赖性。H_2O_2对兔肾小管上皮细胞增殖具有不同程度的影响,肉苁蓉对H_2O_2致兔肾小管上皮细胞损伤具有保护作用。

Rabbit renal tubular epithelial cells were cultured in vitro to study the effect of H_2O_2 on cell proliferation and Cistanche deserticola extract on the tubular epithelial cells injured by H_2O_2.Cell morphology was used to observe the effect of H_2O_2 on the proliferation of rabbit renal tubular epithelial cells by MTT method and the protective effect of 2,20 and 200 mg·L^(-1) C.deserticola water extract and alcohol extract was studied by LDH release.The results showed that the number of morphological changes of cells were gradually increased.The number of attached cells were gradually reduced.The inhibition rate of cell proliferation and LDH release were increased along with increasing concentration of H_2O_2.High dose of C.deserticola extract could significantly decrease the inhibiting effect of RTECs proliferation by H_2O_2(P < 0.01).The water extraction and alcohol extract of C.deserticola could significantly decrease the LDH release(P < 0.01).H_2O_2 had some effects on the proliferation reaction of RTECs of rabbit,while C.deserticola extract has protective effect on RTECs injury by H_2O_2.