异甘草酸镁对乳腺癌细胞系生物学行为的影响及作用机制

Effect and mechanism of magnesium isoglycyrrhizinate on the biological behavior of breast cancer cell lines

目的观察异甘草酸镁对乳腺癌生物学行为的影响,并初步探讨其作用机制。方法将三阴性乳腺癌细胞株MDA-MB-231与ER阳性乳腺癌细胞株MCF-7用不同浓度(0、50、500μmol/L)异甘草酸镁处理,分别采用CCK-8法、划痕实验、LDH活性检测观察异甘草酸镁对两种细胞株细胞增殖、迁移、死亡的作用;利用Western blotting检测异甘草酸镁对细胞不同凋亡蛋白(Bax、Bcl-2、Caspase-3、Caspase-9、HSP70与HSP90)的表达。结果不同浓度异甘草酸镁处理后,乳腺癌细胞的增殖、迁移能力被明显抑制,细胞自噬、凋亡增加;与化疗药物顺铂联合使用后,异甘草酸镁能够提高顺铂对乳腺癌细胞迁移能力的抑制作用。在MDA-MB-231细胞株中,异甘草酸镁能够降低HSP90蛋白的表达,促进Bax蛋白的表达;在MCF-7细胞株中,异甘草酸镁能够降低HSP70蛋白的表达。结论异甘草酸镁对乳腺癌细胞株的生物学行为存在一定抑制作用,与化疗药物顺铂联合使用,能够提高顺铂对肿瘤细胞的抑制作用。异甘草酸镁在MCF-7及MDA-MB-231细胞株中的作用机制存在一定差异。

Objective To explore the effects and mechanism of magnesium isoglycyrrhizinate on the biological behavior of breast cancer. Methods Breast cell lines MDA-MB-231 and MCF-7 were treated with different concentrations （0, 50, 500 μmol/L） of magnesium isoglycyrrhizinate. Cell proliferation, migration and death were detected by CCK-8 assay, wound healing test, and LDH-cytotoxicity assay kit. Effect of magnesium isoglycyrrhizinate on the expressions of Bax, Bcl-2, Caspase-3, Caspase-9, HSP70 and HSP90 in breast cancer cells were examined by Western blotting. Results After treated with different concentrations of magnesium isoglycyrrhizinate, cell proliferation and migration abilities were inhibited in breast cancer cells, while autophagy and apoptosis increased. The inhibitory effects of platinum on cell migration were enhanced by the combination usage with magnesium isoglycyrrhizinate. Expression of HSP90 was reduced and Bax was promoted in MDA-MB-231 cell line. Expression of HSP70 was reduced in MCF-7 cell line. Conclusion Magnesium isoglycyrrhizinate inhibits the malignant phenotype of human breast cancer cell lines and has the synergic antitumor effect combined with platinum. The mechanism of magnesium isoglycyrrhizinate on MCF-7 and MDA-MB-231 cell lines are different.