结肠传输颗粒诊断慢传输型便秘大鼠的实验研究

Experimental Study on the Diagnosis of Slow Transit Constipation Rats with Colon Transport Particles

目的应用洛哌丁胺建立一种大鼠慢传输型便秘模型,并观察结肠传输颗粒在此模型中的传输情况。方法 30只健康SD大鼠随机分为实验组和对照组(n=15)。实验组连续腹腔注射洛哌丁胺21 d,对照组腹腔注射等剂量灭菌注射用水。对比2组大鼠的体质量、24 h排便粒数、粪便湿重、干重、含水率以及口-肛传输时间。造模结束后,将结肠传输颗粒灌胃进大鼠体内,通过不同时间段拍摄大鼠腹部X片,观察其在大鼠体内的传输情况。结果 2组大鼠在进食量与饮水量上差异无统计学意义;造模结束后,2组大鼠在体质量上差异无统计学意义;与对照组相比,实验组大鼠24 h粪便粒数明显减少(P<0.01),粪便湿重(P<0.01)、干重(P<0.05)、含水率(P<0.01)较对照组均显著降低,口-肛传输时间明显延长(P<0.05),差异具有统计学意义;实验组大鼠6,12 h的标志物排出率均显著小于对照组。结论通过使用洛哌丁胺成功建立了慢传输型便秘大鼠模型,并验证了结肠传输颗粒有利于大鼠慢传输型便秘的诊断。

OBJECTIVE To establish a rat model of slow transit constipation by using loperamide, and to observe the transmission of colonic transit particle in this model. METHODS Thirty healthy SD rats were randomly divided into experimental group and control group. In the experimental group, loperamide was injected intraperitoneally for 21 d, and the control group was given the same amount of sterile water for injection. Body weight, the number of 24 h defecation, wet weight, dry weight, water content of faeces, and intestinal transit time were compared among two groups. At the end of the modeling, the colonic transit particles were infused into the rats, then observe its transmission in rats through performing the abdominal X-ray of rats at different times. RESULTS There was no significant difference in the food intake and drinking water between two groups. At the end of modeling, there was no significant difference in body weight between two groups. The number of 24 h defecation in experimental group was significantly lower than that in the control group （P〈0.01）, and the difference in the wet weight （P〈0.01）, the dry weight （P〈0.05） and water content（P〈0.01） of faeces in experimental group were significantly lower than those in control group. The gastrointestinal transit time of the experimental group was significantly longer than that of the control group（P〈0.05）. The rate of discharge of colonic transit particles in experimental group in 6 h and 12 h was significantly smaller than that in control group （P〈0.05）. CONCLUSION The model of rat constipation is established successfully. It was proved that the colonic transit particle is beneficial to diagnose slow transit constioation in rats.