摘要
目的研究刺齿凤尾蕨中的对映-贝壳杉烷二萜类化学成分。方法将刺齿凤尾蕨用95%乙醇提取后,通过有机溶剂萃取,提取液过聚酰胺树脂,中、高压制备液相色谱等多种色谱技术得到单体化合物。运用NMR、ESI-MS等波谱学技术进行结构鉴定。研究各单体化合物对皮质酮诱导PC12细胞损伤的保护作用。结果从该植物提取物中分离并鉴定了10个二萜类化合物,分别为ent-kaurane-16β,17,19-triol-3-one(1),broussonetone A(2),broussonetone B(3),3α-angeloyloxy-17-hydroxyentkaur-15-en^(-1)9-oic acid(4),3α-tigloyloxy-17-hydroxy-ent-kaur-15-en^(-1)9-oic acid(5),tricalysiolide C(6),tricalysiolide D(7),12α-(β-D-glucopyranosyloxy)-7β-hydroxykaurenolide(8),7β-(β-Dglucopyranosyloxy)-12α-hydroxykaurenolide(9)和acantrifoside D(10)。化合物8、9和10对皮质酮诱导PC12细胞损伤具有较强的保护作用。结论化合物1~10,均首次从刺齿凤尾蕨中分离获得。
Objective To investigate the compent of ent-kauranediterpenes from Pteris dispar. Methods The Pteris dispar powder was extracted with 95% EtOH. The dried extract was subjected to polyamide resin and purified by MPLC and preparative HPLC. The structures were established on the basis of extensive spectroscopic analyses, including ESI-MS and NMR. The protective effects of the compounds were tested against corticosterone-induced damage in PC-12 cells. Results Totally 10 alkaloids were isolated from the plant and identified as ent-kaurane-16β, 17, 19-triol-3-one （1）, broussonetone A （2）, broussonetone B （3）, 3a-angeloyloxy-17-hydroxy-ent-kaur-15-en-19-oic acid （4）, 3α-tigloyloxy-17-hydroxy-ent-kaur-15-en-19-oic acid （5）, tricalysiolide C （6）, tricalysiolide D （7）, 12a-（β-D-glucopyranosyloxy）-7β-hydroxykaurenolide （8）, 7β-（β-D-glucopyranosyloxy）-12α-hydroxykaurenolide （9）, and acantrifoside D （10） and compound 8 - 10 had strong protective effect of corticosterone-induced damage in PC-12 cells. Conclusion Compounds 1 - 10 have been obtained from Pteris dispar for the first time.
出处
《中南药学》
CAS
2017年第12期1712-1716,共5页
Central South Pharmacy
基金
黑龙江中医药大学校级基金面上项目(No.21736)
