HPLC-ELSD法测定桔贝合剂中贝母素甲与贝母素乙的含量

Determination of peimine and peiminine in Jubei mixture by HPLC-ELSD

目的建立高效液相色谱-蒸发光散射检测器(HPLC-ELSD)法同时测定桔贝合剂中贝母素甲和贝母素乙含量。方法采用Thermo Hypersil GOLD C_(18)(250 mm×4.6 mm,5μm)色谱柱,乙腈-水-二乙胺(60∶40∶0.03)为流动相,柱温为25℃,流速为1.0 mL·min^(-1);ELSD参数:漂移管温度为85℃,载气流速为2.0 L·min^(-1)。结果贝母素甲在0.572~5.720μg(r=0.9998),贝母素乙在0.795~7.950μg(r=0.9996)与峰面积的对数值呈线性关系;平均加样回收率分别为99.0%(RSD=0.93%)和99.4%(RSD=0.64%)。结论该方法准确可靠、重复性好,为提高桔贝合剂的质量标准提供了科学依据。

Objective To develop an HPLC-ELSD method for the simultaneous determination of peimine and peiminine in Jubei mixture. Methods The analysis was performed on a Thermo Hypersil GOLD C18 column （250 mm × 4.6 mm, 5 μm）, the mobile phase consisted of acetonitrile-water and diethylamine （60 ：40 ： 0.03）, the flow rate was 1.0 mL · min-1, and the column temperature was 25 ℃ ; ELSD parameters： the temperature of drift tube was 85 ℃ and the carrier gas flow rate was 2.0 L · min-1. Results The linear ranges of peimine and peiminine were 0.572 - 5.720 μg （r = 0.9998） and 0.795 - 7.950 μg （r = 0.9996）, respectively. The average recovery for peimine and peiminine was 99.0% （RSD was 0.93%） and 99.4% （RSD was 0.64%）. Conclusion The method is accurate, reliable and reproducible, and provides a scientific basis for improving the quality standard of Jubei mixture.