生物陶瓷材料iRoot BP Plus对小型猪修复性牙本质形成影响的实验研究

Evaluation of bioceramic iRoot BP Plus as a pulp capping agent in swine

目的应用小型猪实验动物模型评价iRoot BP Plus直接盖髓后修复性牙本质桥形成的情况以及牙髓的生物学反应。方法选取4只18月龄小型猪前磨牙26颗,制备直径为2mm穿髓孔,随机分3组:氢氧化钙组、MTA组和iRoot BP Plus组,进行直接盖髓。术后8周,组织病理学观察。结果 MTA组和iRoot BP Plus组均可以形成具有天然牙本质小管样结构及极性的成牙本质细胞层的钙桥。与MTA组相比,iRoot BP Plus组形成的钙桥质地较为均匀连续,牙髓组织炎症反应较轻,髓腔未见不规则钙化。氢氧化钙组表现钙桥较为疏松,冠部牙髓组织坏死,根髓可见不同程度的炎症反应,根管壁出现不规则钙化。结论 iRoot BP Plus和MTA盖髓明显优于传统的氢氧化钙。iRoot BP Plus在盖髓术中表现了良好的生物学特性。

Objective To investigate the formation of reparative dentin bridge and the dental pulp response to the pulp capping agent iRoot BP Plus in miniature swine. Methods Twenty-six premolars of 4 miniature swine were randomly assigned into three groups. Class I cavities with 2mm diameter of pulp exposures were prepared on the occlusal surfaces. Two months later, teeth were harvested, fixed and processed for histological analysis. Results In MTA and iRoot BP Plus group, homogenous dentinal tubule-like structure of reparative dentin formed. There was only slight inflammatory response and no obvious calcification in the iRoot BP Plus group. In calcium hydroxide group, pulp necrosis occurred underneath the materials. The newly formed calcium bridge was loose. Pulp chamber showed vascular congestion, infiltration of inflammatory cell and irregular calcification. Conclusion iRoot BP Plus calcium hydroxide is a good candidate for maintaining pulp tissue.