STEAP1在人乳腺癌中表达及其临床意义

Expression of STEAP1 in human breast cancer and its clinical significance

目的:研究前列腺跨膜上皮抗原1(six-transmembrane epithelial antigen of prostate protein 1,STEAP1)在乳腺癌中的表达及临床意义。方法:收集2007年1月至2016年12月山东省千佛山医院40例女性乳腺正常组织及52例乳腺纤维腺瘤、211例乳腺癌患者的组织标本,应用免疫组织化学法检测STEAP1的表达情况。根据STEAP1表达的高低将乳腺癌患者分为高表达组和低表达组,对两组行临床病理特征和Kaplan-Meier生存分析。运用RT-PCR及Western blot法检测STEAP1在人正常乳腺上皮细胞HBL-100及乳腺癌细胞MDA-MB-468、BT549和MCF-7中的表达情况,使用Oncomine和Kaplan-Meier Plotter数据库分析STEAP1的表达及预后。结果:免疫组织化学法检测显示乳腺癌组织中的STEAP1高表达者占11.8%(25/211),低于乳腺正常组织的85.0%(34/40)和乳腺纤维腺瘤的84.6%(44/52),3者之间具有显著性差异(P<0.001),与Oncomine数据库分析获得的乳腺癌组织中表达低于乳腺正常组织的结果一致。RT-PCR和Western blot法检测显示乳腺上皮细胞中的STEAP1 m RNA和蛋白质表达明显高于乳腺癌细胞MDA-MB-468、BT549和MCF-7,4种细胞之间具有显著性差异(均P<0.001)。Kaplan-Meier生存分析与Kaplan-Meier Plotter数据库分析均表明STEAP1高表达者预后更佳(均P<0.05)。结论:正常乳腺中的STEAP1表达水平高于乳腺癌,STEAP1高表达与良好的预后相关。

Objective：To investigate the expression of STEAP1 in human breast cancer and its clinical significance.Methods：Immunohistochemical staining was performed to detect the expression of STEAP1 in breast tissues from 40 normal women,52 patients with breast fibroadenoma,and 211 patients with breast cancer in Qianfo Hill Hospital of Shandong Province from January 2007 to December 2016.According to the expression level of STEAP1,the breast cancer patients were assigned into high and low expression groups.Clinical pathological characterization and Kaplan-Meier survival analysis of the two groups were performed.RT-PCR and Western blot were conducted to measure the expression of STEAP1 in mammary gland cells.The Oncomine and Kaplan-Meier Plotter databases were used to analyze the expression and prognosis of STEAP1.Results：Immunohistochemistry showed that the high expression rate of STEAP1 in breast cancer was 11.8%（25/211）,which was significantly lower（P0.001） than that in normal breast（85.0%,34/40） and fibroadenoma（84.6%,44/52）.The result was consistent with the Oncomine database analysis that the expression in breast cancer tissue was lower than that in normal breast tissue.RT-PCR and Western blot analyses showed that the m RNA and protein expression levels of STEAP1 were significantly higher in mammary epithelial cells than in MDA-MB-468,BT549 and MCF-7 breast cancer cell lines,and a significant difference was found among the four types of cells（P0.001）.Kaplan-Meier survival analysis and Kaplan-Meier Plotter database analysis showed that patients with high STEAP1 expression had better prognosis than those with low STEAP1 expression（P0.05）.Conclusion：The expression level of STEAP1 in normal breast was higher than that in breast cancer.High STEAP1 expression is associated with a better prognosis.