摘要
2015年,在陕西榆林地区发现一种新的荞麦叶部病害,发病叶片上出现坏死斑,中间浅褐色,边缘褐色,外围有褪绿晕圈,发病严重时叶片枯死。为明确引起该病害的病原菌种类,采用组织分离法分离和纯化得到真菌分离物。通过柯赫氏法验证、形态学观察和rDNA-ITS序列分析(GenBank注册号:LC208725.1),确定该病害病原菌为荞麦派伦霉叶斑病菌(Peyronellaea calorpreferensi,又名Didymella heteroderae)。这是派伦霉所致荞麦叶斑病在中国的首次报道。
In 2015, a new disease of leaf spot in buckwheat was observed in Yulin, Shaanxi Province, which could cause necrosis with brown edge, and it was surrounded by pale green ring. The heavy diseased leaves turned yellow and died. The pathogen strain was isolated and purified by tissue isolation method. Based on morphological observation, sequence analysis of rDNA ITS （GenBank Accession No. LC208725.1）, and Koch’s postulate, the pathogen was identified as Peyronellaea calorpreferensi, also named Didymella heteroderae. P.calorpreferensi infecting buckwheat was reported first time in China.
出处
《西北农业学报》
CAS
CSCD
北大核心
2017年第10期1544-1549,共6页
Acta Agriculturae Boreali-occidentalis Sinica
关键词
荞麦
派伦霉属
形态特征
分子鉴定
Buckwheat
Peyronellaea calorpreferensi
Morphological characters
Molecular identification
