超敏蛋白BaPE1对烟草马铃薯Y病毒的诱导抗性

Effects of Harpin BaPE1 on PVY-resistant of Tobacco

为了探究超敏蛋白对烟草马铃薯Y病毒病的诱导抗性影响,采用半叶枯斑法和田间效果试验研究超敏蛋白BaPE1提取物对枯斑三生烟和普通烟的诱导抗性和促生作用。结果表明,超敏蛋白BaPE1能诱导烟株对烟草马铃薯Y病毒产生抗性,并对烟草有一定的促生作用。接种前48h喷施500μg/mL超敏蛋白BaPE1预防效果达70.46%,接种后24h、48h喷施500μg/mL超敏蛋白BaPE1治疗效果达61.30%和60.59%,均显著高于对照组;喷施500μg/mL超敏蛋白后烟草在株高、茎围、最大叶面积等方面表现出较好的生长势,并高于其他处理。因此,超敏蛋白BaPE1不仅可以提高烟草抗性而且还能促进烟株生长、提高烟叶经济效益。

In order to study effect of Harpin BaPE1 on induced resistance on PVY, the half leaf method and field experiment were selected to detect the anti PVY activity and growth promoting effects of Harpin BaPE1 on Nicotiana tabacum var.‘Samsun NN’ and N.tabacum.The results showed that the Harpin BaPE1 induced the tobacco got induced resistance and growth promoting. Spraying 500 μg/mL Harpin 48 h before PVY inoculation, the prevention effect was up to 70.46%, and the treatment effect of 24 h,48 h after inoculation were 61.30% and 60.59%, and all the effects were significantly higher than the control. After spraying 500 μg/mL Harpin, the tobacco performed better growth potential in plant height, stem girth and area of maximum leaf, and higher than the other treatments. Therefore the Harpin BaPE1 not only could enhance the tobacco resistance of PVY, but also promote the growth.