莫诺苷对慢病毒载体转染抑制人胚胎神经干细胞增殖能力的影响

Effects of Morroniside on Lentivector Induced the Inhibition of Human Embryonic Neural Stem Cells Proliferation

目的人胚胎神经干细胞(Human embryonic neural stem cell,he NSC)具有自我更新能力和多向分化潜能,莫诺苷具有促进脑缺血再灌注损伤神经功能恢复的作用,但其对he NSC的研究未见报道。方法本研究建立慢病毒载体转染he NSC模型,给予100μmol/L莫诺苷作用48 h,观察对干细胞增殖的影响。结果与正常细胞比较,转染慢病毒载体后,he NSC Ki67+细胞数量以及Brd U+细胞数量均显著下降(P<0.01,P<0.001);而与莫诺苷共孵育后,转染慢病毒载体的he NSC Ki67+细胞数量以及Brd U+细胞数量均显著增加(P<0.05,P<0.001)。结论慢病毒载体转染能抑制he NSC增殖能力,而莫诺苷能显著改善慢病毒载体引起的细胞增殖能力下降。

Objective Human embryonic neural stem cells （heNSCs） have potential capacity of self-renewal and multi-lineage differentiation, which brings new hope for nervous system diseases treatment, although its biological properties and differentiation regulation mechanism is not yet clear. Method Lentiviral vector stands out due to its good performance,providing a new pathway new hope to not only the research on gene function, but also the gene therapy of precision medicine. Result The hNSCs were transduced with lentiviral vector, and then were incubated with Morroniside at the dose of 100 μmol. L-1. Cell proliferation were detected by Ki67 staining and BrdU staining. Compared with the normal group, the number of Ki67 ＋/DAPI ＋ and BrdU ＋/DAPI ＋ cells in lentivector groups were decreased significantly （P 〈 0.01, P 〈 0. 001 ）. After treatment with Morronside, compared with lentiveetor group, the number of Ki67 ＋/DAPI＋ and BrdU ＋/DAPI ＋ cells were increased significantly （ P 〈 0. 05, P 〈0. 001 ）. Conclusion The result suggested that the proliferation of neural stem cells were affected by transfected with lentiviral vector, but Morroniside can reverse the inhibition of proliferation caused by lentivector.