摘要
传统胚胎冷冻技术如OPS、冷冻环、冷冻帽、冷冻膜等,在胚胎复苏率和囊胚形成率、技术可行性、温度适用范围、冰冻和解冻速率等方面各具有其特定优缺点,在实际推广和应用中存在一定局限性。本文就新型微滴式玻璃化(Modified droplet vitrification,MDV)胚胎冷冻技术和高重量渗透摩尔浓度玻璃化(High osmolality vitrification,HOV)两种新型胚胎冷冻保存技术进行概述,并阐明胚胎冷冻保存及复苏发育过程中所受温度、饮食中植物雌激素、N-乙酰半胱苷酸等参数的影响。
Most conventional ultra-rapid cooling vitrification techniques have unique advantages and disadvantages on survival rate, developmental potential, technical feasibility, transport costs, warming speed, such as electron microscope grids, open pulled straws, Cryoloop, Cryotech,Cryotops, Cryotip, Cryotube, or thin plastic strips. This article reviews modified droplet vitrification and high osmolality vitrification, illustrates the role of diet, housing- temperature and N-acetyl cysteine in the productionof genetically modified mouse embryos and their developmental capacity after cryopreservation
出处
《实验动物科学》
2017年第6期61-64,共4页
Laboratory Animal Science
基金
<超低温保存模型动物胚胎服务平台建设>项目(PXM2015_178102_000002_00178761_XMCG)
关键词
胚胎冷冻
MDV
HOV
胚胎发育
cryopreservation
modified droplet vitrification
high osmolality vitrification
embryo production
