病毒性肝炎及肝癌患者血清GP73的表达及意义

Expression and clinical significance of serum GP73 in patients with virus hepatitis and liver cancer

目的探讨血清高尔基体蛋白73(GP73)在病毒性肝炎及肝癌患者中的表达及临床意义。方法病例资料来源于2013-2015年首都医科大学附属北京同仁医院。应用双抗体夹心ELISA方法测定192例健康对照组、118例乙型肝炎组、131例丙型肝炎组和78例肝癌组患者的血清GP73水平。结果乙型肝炎组、丙型肝炎组和肝癌组患者的GP73浓度均明显高于健康对照组,差异均有显著统计学意义(P<0.01);在乙型肝炎患者中,HBe Ag阳性组GP73浓度和HBV-DNA含量明显高于HBe Ag阴性组,差异均有显著统计学意义(P<0.01);肝癌组患者的GP73与甲胎蛋白(AFP)呈正显著相关性(r=0.433,P<0.01)。结论与健康对照组比较,GP73在不同肝炎组及肝癌组患者中均明显增高,且随着疾病进展,GP73有明显增高趋势,表明血清GP73测定在病毒性肝炎及肝癌的诊断及监测疾病进程中具有重要意义。

Objective To investigate the expression and clinical significance of serum Golgi protein-73（GP73）in healthy controls and patients with virus hepatitis and liver cancer. Methods Serum samples were obtained from patients who admitted to Beijing Tongren Hospital, Capital Medical University from 2013 to 2015. By Enzyme-linked immunosorbent assay（ELISA）, serum GP73 was measured in 192 healthy controls, 118 patients with HBV infection, 131 patients with HCV infection and 78 patients with liver cancer. Results The GP73 value in different liver diseases group was significantly higher than that in the healthy controls, respectively（P〈0.01）. The GP73 value and quantitative HBV DNA of HBe Ag-positive group were significantly higher than those of HBe Ag-negative group, respectively（P〈0.01）.GP73 had the significant positive correlation with AFP in liver cancer group（r=0.433, P〈0.01）. Conclusion Compared with the healthy controls, GP73 in patients with different liver diseases is significantly increased. These results suggested that GP73 might play an important role in the diagnosis of liver diseases and monitor of disease progression.