壳聚糖-岩藻聚糖硫酸酯纳米粒的制备及性质研究

Preparation and property of chitosan-fucoidan nanoparticles

目的以低分子量岩藻聚糖硫酸酯为交联剂,研究了聚电解质凝聚法制备壳聚糖-岩藻聚糖硫酸酯纳米微粒(Chitosan-fucoidan nanoparticles,CS-Fuc NPs)的制备工艺,并对纳米粒的胃肠道和贮藏稳定性进行研究。方法采用聚电解质凝聚法制备壳聚糖-岩藻聚糖硫酸酯纳米微粒,采用体外模拟胃液和模拟肠液消化体系,检测纳米粒的胃肠稳定性,常温贮藏实验测定纳米粒的短期贮藏稳定性。结果壳聚糖-岩藻聚糖硫酸酯纳米微粒的最优制备条件是:质量分数0.1%岩藻聚糖硫酸酯与质量分数0.1%壳聚糖体积比为1.1∶1,pH 4.5,温度30℃。所得纳米粒粒径为227.8nm,Zeta电位为38.4mV,PDI为0.231,岩藻聚糖硫酸酯复合率(Fuc复合率)为94.92%。所制备的纳米颗粒在10周内没有显著变化,在模拟胃环境中稳定性良好,在模拟肠道环境中解聚性良好。结论壳聚糖-岩藻聚糖硫酸酯纳米粒制备工艺简单,性能良好,有望成为新型的口服药物运送载体。

Objective To optimize the polyelectrolyte complexation technology of chitosan-fucoidan nanoparticles by using the low weight molecular fucoidan as a crosslinking agent and study its gastrointestinal stability and storage stability.Methods Polyelectrolyte complexation was used to prepare the nanoparticles.Gastrointestinal stability of the nanoparticles was measured by in vitro simulated digestion and storage stability was determined by storage at 25℃.Results When the fucoidan/chitosan ratio was 1.1/1,temperature was 30℃ and pH was 4.5,the optimum preparation conditions were achieved.The optimum nanoparticles had an average diameter of 227.8 nm,a Zeta potential of 38.4 mV,a PDI of 0.231 and a fucoidan loading rate of 94.92%.The chitosan-fucoidan nanoparticles demonstrated no significant changes within 10 weeks,and they were stable in simulated gastric environment and had a good depolymerization in simulated intestinal environment.Conclusion The chitosan-fucoidan nanoparticles have a simple preparation process and good nature,it could be used as a new carrier for oral drugs.