应用东乡野生稻回交重组自交系群体分析糙米矿质含量QTL

QTL Analysis for Mineral Contents in Brown Rice Using a BC_2F_(4:5) Population Derived from Dongxiang Wild Rice(Oryza rufipogon Griff.)

【目的】强化粮食作物的必需矿物质有利于缓解人们矿质营养缺乏症。【方法】从协青早B//协青早B/东乡野生稻BC_1F_5群体中挑选到1个单株A58,与协青早B回交,构建了BC_2F_(4:5)群体。采用电感耦合等离子体原子发射光谱仪ICP-AES测定132个BC_2F_(4:5)株系的糙米Mg、Ca、Zn、Fe、Mn和Cu含量,应用Windows QTL Cartographer 2.5进行糙米矿质QTL分析。【结果】共检测到17个糙米矿质含量QTL,分别位于第1、4、6、8、9和11等6条染色体上,包括Mg含量1个、Ca含量4个、Zn含量4个、Fe含量2个、Mn含量2个和Cu含量4个。这些QTL解释表型变异的5.0%~47.2%,其中8个QTL的增效等位基因来自东乡野生稻。【结论】12个QTL聚集于5条染色体上的5个QTL簇,表明不同矿质营养元素涉及到共同遗传生理机制,可通过分子标记辅助选择方法将有利等位基因应用于稻米营养品质改良。

【Objective】Biofortifying food crops with essential minerals would alleviate mineral deficiencies in humans.【Method】One plant A58 was selected from Xieqingzao B// Xieqingzao B /Dongxiang wild rice BC1F5 population, and was backcrossed with Xieqingzao B to develop a BC2F4：5 population. The contents of Mg, Ca, Zn, Fe, Mn and Cu in brown rice of 132 BC2F4：5 lines were measured with an inductively coupled plasma atomic emission spectrometer （ICP-AES）. Detection of quantitative trait loci （QTLs） for mineral contents in brown rice was conducted using Windows QTL Cartographer 2.5.【Result】A total of 17 QTLs for mineral contents in brown rice were detected on chromosomes 1, 4, 6, 8, 9 and 11, respectively, including one for Mg content, four for Ca content, four for Zn content, two for Fe content, two for Mn content, and four for Cu content. The explained phenotypic variations ranged from 5.0% to 47.2%, and eight QTLs of them had the enhancing alleles derived from Oryza rufipogon. 【Conclusion】Twelve QTLs were clustered in five chromosomal regions, indicating that common genetic-physiological mechanisms were involved for different mineral nutrients, and the beneficial alleles could be utilized to improve grain nutritional quality by marker-assisted selection.