体外试验中医药膳血清样品制备的实践经验体会

Experience of preparing medicated food-serum sample in vitro experiment

含药血清是探讨药膳作用机制体外试验的主要干预手段。本文从实践国家自然科学基金课题"从TLR4/NF-κB信号通路探讨芪药鸡金粥调控5-FU所致胃肠黏膜损伤肠道紧密连接蛋白表达的机制"(编号81403446)的体外试验,积累"芪药鸡金粥"含药血清样品制备保存的经验,现从动物准备、给药方案、采血时机、血清灭活储存等方面进行报道,旨在为将来在中医护理领域中从事相关研究的科研工作者提供参考与借鉴。

The preparation of medicated serum is one of the key procedures fomechanisms of medicated food in vitro experiment. Based on the National Natural Science Fdation project entitled “Effect of medicated food QiYaoJiJinZhou for Tight Junction Proteins in 5-FU induced Gastrointestinal Mucositis： Role of TLR4/NF- kB Pathway” （No. 81403446）. This paper summarized the experience of preparing medicated food-serum sample in vitro experiment. Five aspects were introduced in this paper,including the selection of animals,identification ofdosage regimen,identification of appropriate time point for taking blood sampling,inactivationand storage of serum and study limitations and implications. This paper intended to provide soimplications for researchers who are interested in similar research topics in the areas of traditionalmedicine nursing.