多孔丝素颗粒的可控制备及药物负载研究

Size-controlled preparation of porous silk fibroin particle used as drug carrier

近年来桑蚕丝素蛋白以来源广泛、可降解且生物相容性好等优点,在生物医用材料领域得到了越来越广泛的关注。以丝素蛋白为原材料,研究丝素蛋白浓度、熟化温度及盐浴浓度对所制备的丝素蛋白粒径及形貌的影响,并探索该颗粒对抗肿瘤药物阿霉素的负载和控释能力。研究结果表明:丝素颗粒表面呈多孔状态的球体,粒径变化范围在400~3600nm,对药物阿霉素的包封率为93.86%,载药量为14.08μg/mg;随pH降低,药物释放速率和累积释放量增加。实验表明:纳米级丝素颗粒可作为药物载体,可高效包覆抗癌药物阿霉素,并实现药物的可控释放。

Recently,silkworm silk fibroin has received more and more attention in the field of biomedical material due to its excellent properties,such as extensive sources,biodegradability and biocompatibility.In the paper,the silk fibroin(SF)was used as the raw material to study the effects of SF concentration,aging temperature and salt bath concentration on the size and morphology of SF particles.Meanwhile,the loading capacity and controlled release capacity of the particles on anti-tumor drug were explored.Results show that the synthesized SF particle is spherical and porous.The diameter of SF particle could be adjusted in a range of 400~3600 nm.The loading content and the entrapment ratio of doxorubicin are14.08μg/mg and 93.86%respectively.The release rate and accumulative release amount of drug increases with the decrease of pH.It is indicated that the SF particles can be used as the drug carrier to effectively coat the anti-cancer drug adriamycin.Besides,controlled drug release can be achieved.