虫酰肼和灭幼脲对锈色粒肩天牛氧化酶和解毒酶活性的影响

Oxidase and detoxifying enzyme activity of Apriona swainsoni(Hope)with diflubenzuron and flubenzuron

为探究虫酰肼和灭幼脲对锈色粒肩天牛Apriona swainsoni(Hope)生理机制的影响,用经灭幼脲(T1)和虫酰肼(T2)处理的木屑饲喂锈色粒肩天牛幼虫,测定天牛幼虫的过氧化氢酶(CAT),超氧化物歧化酶(SOD),谷胱甘肽S转移酶(GSTs)和羧酸酯酶(Car E)活性,每12 h取样,持续72 h。结果显示:T1处理CAT活性先升高,24 h后降低;T2处理CAT活性降低;与对照组相比,T1和T2处理SOD活性、GSTs活性显著增高(P<0.05),Car E活性则无明显差异。推测锈色粒肩天牛幼虫GSTs活性和虫体抗性的产生有关,GSTs活性可能是天牛幼虫的抗性标识物。

To research the influence of tebufenozide and chlorbenzuron on the physi ological mechanism of Apriona swainsoni（Hope）, A. swainsoni larvae were fed with sawdust treated by chlorbenzuron（T1） and tebufenozide（T2）. These were then sampled to determine enzymatic activity of catalase（CAT）, superoxide dismutase（SOD）, glutathione S transferases（GSTs）, and carboxylesterase（Car E） in larvae at intervals of 12 h to 72 h in succession. Results showed that after treatment with chlorbenzuron, the enzymatic activity of CAT increased first and then decreased 24 h later. Enzymatic activity of SOD maintained a high level. Also, after treatment by tebufenozide, the enzymatic activity of CAT decreased first with enzymatic activity of SOD being higher than the control. After treatment by two types of the pesticides, enzymatic activity of GSTs was higher than the control with no differences of Car E activity with the control. Therefore, enzymatic activity of GSTs in A. swainsoni was assumed to be associated with resistance generated in the larva and was likely to be a resistance marker of the larvae.