粪样保存方法对黑白仰鼻猴肠道结节线虫监测的影响

Effects of Fecal Sample Preservation Methods on the Monitoring of Intestinal Oesophagostomum in Rhinopithecus bieti

目的:探索一种有效的黑白仰鼻猴肠道寄生虫虫卵定量监测的粪便样品保存方法。方法:对两种保存方法(福尔马林保存液保存和冷冻保存)下的黑白仰鼻猴粪便样品进行寄生虫虫卵检出率及检出强度进行对比检测,分析不同保存条件下的粪便保存效果。结果:-20℃冷冻条件下保存的粪便样品,结节线虫虫卵检出强度为(919.69±916.09)个/g,检出率是88.89%(56/63),检出率在30 d内没有下降,检出强度也维持在较高的水平上。福尔马林液保存的粪便样品在第4天时结节线虫的检出强度就下降至4.98%,检出强度及检出率都呈现显著的差异(t=6.799,P<0.001,df=62;χ2=45.10,P<0.01)。结论:将黑白仰鼻猴粪便置于-20℃冷冻条件下保存,在保证结节线虫检出率及检出强度的情况下,可有效延长保存时间。

Objective： To explore an effective fecal sample preservation method for quantitative monitoring of intestinalOesophagostomum in Rhinopithecus bieti. Methods： The detection rate and detection intensity of Oesophagostomum in Rhinopithecus bieti fecal samples from two preservation methods（formalin preservation and cryopreservation）were compared and analyzed, and thesample preservation under different storage conditions was analyzed for different effects. Results： The samples stored at-20 ℃ showedthat the detection intensity of Oesophagostomum eggs was（919.69 ± 916.09）/g, the detection rate was 88.89%（56/63）, the detectionrate did not decline within 30 days, and the detected intensity also maintained at a high level. The sample stored in formalin reducedits intensity to 4.98% on 4 th day, and the detection intensity and detection rate showed significant differences（t=6.799, P 〈0.001, df=62; χ2=45.10, P0.01）. Conclusion： In a frozen condition at-20 ℃ which ensures the detection rate and intensity of Oesophagostomum,the storage time of the fecal samples of Rhinopithecus bieti can be effectively prolonged.