应激条件下催乳素释放机理的探讨

Roles of Oxytocin,Serotonin and Norepinephrine in Regulation of Prolactin Release during Stress

本文对可能的催乳素(PRL)释放促进因素催产素(OT)、去甲肾上腺素(NE)和5-羟色胺(5-HT)在应激条件下的PRL释放作用进行了初步探讨。观察到第三脑室注射抗OT抗体,可使应激引起PRL升高的幅度下降;第三脑室注射6-羟多巴胺(6-OHDA)可消除应激引起PRL的升高,而第三脑室注射二甲麦角新碱(methysergide,MSG)后,血浆PRL明显升高,给MSG加应激未见进一步升高PRL;第三脑室注射5,6-双羟色胺(5,6-DHT)对应激导致的血浆PRL升高无明显影响。结果提示:OT和NE可能参与应激状态下PRL释放的调节。

The stimulating effects of oxytocin (OT), norepinephrine (NE) and seroto- nin on prolactin (PRL) release in vitro and in vivo have been well documented. In this paper. experiments were made to further demonstrate their roles in regulation of restraint stress--induced PRL release. Male Sprague--Dawley rats were used and carefully handled according to all requirements for stress experiments. Cerebral injection of anti--OT antiserum and 6--OHDA significantly attenuated the increase of PRL during stress, where- as 5, 6--dihydroxytryptamine appeared to have no effect on stress--induced in- crease of PRL. Plasma PRL in animals injected with methysergide showed that the stress increased even more markedly as compared with control animals. However, there was no difference between methysergide--injected animals with and without stress. The results indicate that OT as well as NE may play an important role in stress--induced PRL release, while the serotoninergic (5--HT) system may not.