响应面法优化超声辅助提取黄秋葵花总黄酮的工艺研究

Optimization of ultrasonic-assisted extraction of total flavones from okra flowers by response surface methodology

目的采用响应面法对超声辅助提取黄秋葵花总黄酮的工艺进行优化。方法在提取剂浓度、液料比例、提取温度、水浴提取时间、超声波提取时间试验结果的基础上,采用Box-Behnken design试验设计原理,设计4因素3水平试验,以响应面分析法优化液料比例、乙醇浓度、提取温度、水浴提取时间4个因素对黄秋葵花总黄酮提取率的影响。结果黄秋葵花总黄酮提取率的最佳工艺条件为:液料比例160:1(mL:g)、乙醇浓度42%、提取温度80℃、水浴提取时间16 min和超声提取时间30 min。在此条件下,黄秋葵花总黄酮的提取率为(2.487±0.05)%,真实值与模型预测值相对误差为1.23%。结论该提取工艺提高了黄秋葵花总黄酮的提取率,为其开发利用提供理论支持。

Objective To optimize the ultrasonic-assisted extraction of total flavones from okra flowers by response surface methodology. Methods Based on the results of extraction concentration, ratio of solvent to material, extraction temperature, water bath extraction time and ultrasonic extraction time experiment, the Box-Behnken design method was used to conduct the 4-factors and 3-levels experiment on the basis of single-factor responsive surface analysis, with the yield of total flavones as response value, and the extraction conditions including the ratio of solvent to material, ethanol concentration, extraction temperature and water bath extraction time as response factors. Results The optimal preparation process of ultrasonic extraction for total flavones from okra flowers were as follows： 160：1 （mL：g） of solvent to material, 42% ethanol concentration, 80 ℃ of extraction temperature, 16 min of water bath extraction time and 30 min of ultrasonic extraction time. Under this condition, the extracting rate of total flavones from okra flowers was （2.487±0.05）% and the relative error of real value and model prediction value was 1.23%. Conclusion The extraction process improves the extraction rate of total flavones from okra flowers, which provides theoretical support for its development and utilization.