猪FRZB、ABCF1基因多态性与生长性状的关联分析

Genetic Polymorphism of FRZB Gene and ABCF1 and Their Association with Growth Traits in Pigs

FRZB基因通过抑制Wnt信号途径调控细胞生长,ABCF1(也称为ABC50)结合e IF2,在翻译起始中发挥着重要的作用,可作为猪生长性状相关的候选基因。本研究采用直接测序法检测FRZB基因和ABCF1基因5'非翻译区中单核苷酸多态性(SNPs)基因型分布,并分析基因型与生长性状的相关性。结果表明:在美系大白猪中,FRZB基因位点ins(A-532G)多态性与背膘厚呈极显著相关(P<0.01),与体长接近显著水平(P=0.05);ABCF1基因254A/G多态性与背膘厚、左乳头数和右乳头数均呈显著相关(P<0.05);在法系大白中,ABCF1基因254A/G多态性与初生重呈显著相关(P<0.05)。结果表明,FRZB和ABCF1基因是潜在的影响猪生长性状的候选基因。

FRZB gene regulates cell growth by inhibiting Wnt signaling pathway, ABCFI （ also known as ABC50： ATP- binding cassette 50） binds to eukaryotic initiation factor 2 （eIF2） and is required for efficient translation initiation, so they can be selected as candidate genes for growth trait. In this study, we detected the genotype of two SNPs in 5 ＇ -UTR of FRZB and ABCFlgene by sequencing in pig population and analyzed the effects of the genotype on growth traits. The results showed that the locus ofA-532G ofFRZB gene in American Large White pigs was significantly associated with back fat thickness （P〈0.01）, and the body length was closed to significant level （P=0.05）. For the locus of 254A/G ofABCF1 gene, there were significantly associated with the back fat thickness, left and right nipple of teat number in American Large White pigs （P〈0.05） , and the birth weight in France Large White pigs （P〈0.05）. These results suggest that FRZB and ABCFI may be the major candidate genes for growth traits in pigs.