家兔HSL基因多态性及其与生产性状关联性分析

Polymorphism of HSL Gene and Its Association with Production Traits in Rabbits

本实验采用PCR-SSCP方法对齐卡巨型白兔和齐兴肉兔HSL基因外显子1的遗传多态性进行研究,并进一步分析HSL基因对生产性状的遗传效应。结果表明:HSL基因外显子1第784位处发生了碱基突变(A→C),共出现AA、AB和BB 3种基因型,在2个品种兔群体中AA型均为优势基因型,A均为优势等位基因;卡方适合性检验表明,2个品种兔群体均显著偏离哈代-温伯格平衡(P<0.05);在齐卡巨型白兔中,HSL基因AA型和AB型个体的宰前活体重均显著高于BB型个体(P<0.05),AA、BB型个体的全净膛屠宰率和半净膛屠宰率均显著低于AB型个体(P<0.05);在齐兴肉兔中,HSL基因AA型个体的宰前活体重显著高于AB、BB型个体(P<0.05),BB型个体的全净膛屠宰率和半净膛屠宰率均显著低于AA、AB型个体(P<0.05);在2个品种兔群体中,不同基因型个体的滴水损失和剪切力差异均不显著(P>0.05)。

The polymorphisms of the exon 1 of HSL gene in ZIKA rabbits and Qixin rabbits were analyzed by PCR-SSCP. And the genetic effects of HSL gene on production traits were investigated. The results showed that the single base was changed on 784st spot of the exon 1 ofHSL gene （A→C） and 3 genotypes（AA, AB, BB） were detected. AA was predominant genotype and A was the predominant allele in this two rabbit breeds. The Z2 test indicated that ZIKA rabbits and Qixin rabbits were not in Hardy-Weinberg equilibrium at this SNPs （P〈0.05）. In ZIKA rabbits, Liveweight before slaughtering of individuals with AA and AB genotype were significantly higher than that with BB genotype（P〈0.05）; the slaughter rate and half carcass percentage of individuals with AA and BB genotype were significantly lower than that with AB genotype（P〈0.05）. In Qixin rabbits, Liveweight before slaughtering of individuals with AA genotype were significantly higher than that with AB and BB genotype（P〈0.05）, the slaughter rate and half carcass percentage of individuals with BB genotype were significantly lower than that with AA and AB genotype（P〈0.05）. There was no significant difference on the drip loss and shear force among different genotyoe individuals in this two rabbit breeds （P〉0.05）.