MrMYB1-MrbHLH1：一个有潜力的园艺植物转基因可视化报告基因

MrMYB1-MrbHLH1,a Potential Visible Reporter Gene in Horticultural Plant Transformation

将杨梅果实中参与花色苷生物合成的转录因子编码基因MrMYB1和MrbHLH1同时搭载到双元表达载体的多克隆位点内,重组成双价表达载体,通过农杆菌介导的叶盘法将其分别转入烟草、番茄和森林草莓,成功转化MrMYB1-MrbHLH1的烟草、番茄和森林草莓不定芽因大量积累花色苷而呈红色,与野生型或假阳性不定芽相比,表型差异明显;转化35S::MrMYB1-MrbHLH1的烟草和番茄植株中可显著积累花色苷呈红色,且该性状可稳定遗传。MrMYB1-MrbHLH1在3种园艺植物具有调控花色苷积累而使阳性转化植物部分组织器官呈现红色的功能,是1个有潜力的遗传转化可视化报告基因。

The binary vector,contained both MrMYB1 and MrbHLH1,which were related to anthocyanin biosynthesis regulation in Chinese bayberry was recombined. Subsequently, it was transformed into tobacco,tomato and strawberry,respectively. The 35 S：：MrMYB1-MrbHLH1 lines of these different species were successfully obtained through Agrobacterium-mediated transformation, with accumulation of anthocyanin and red buds,while the wild type plants have the green buds. Furthermore,anthocyanin accumulations were also observed in various tissues of the transgenic tobacco and tomato plants,which are hereditable. Taken together,MrMYB1-MrbHLH1 has the conserved function for anthocyanin biosynthesis in three kinds of horticultural plants and exhibited the potential to be used as a visible reporter gene in plant transformation.